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KMID : 1094020150320030224
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Journal of Veterinary Clinics
2015 Volume.32 No. 3 p.224 ~ p.230
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Chitosan-alginate Gel Modified Poly (L-Lactic-co-ε-Caprolactone) (PLCL) as a Scaffold for Cartilage Tissue Engineering
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Bibek Chandra Sutradhar
Hwang Ya-Won
Choi Seok-Hwa
Kim Gon-Hyung
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Abstract
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This study was designed in the fabricated poly (L-Lactic-co-ε-Caprolactone) (PLCL) scaffold using chitosanalginatehydrogel, which would be more suitable to maintain the biological and physiological functions continuingthree dimensional spatial organizations for chondrocytes. As a scaffold, hydrogels alone is weak at endure complexloading within the body. In this study, we made cell hybrid scaffold constructs with poly (L-Lactic-co-ε-Caprolactone)(PLCL) scaffold and hydrogels to make a three-dimensional composition of cells and extracellular matrix, which wouldbe a mimic of a native cartilage. Using a particle leaching technique with NaCl, we fabricated a highly-elastic scaffoldfrom PLCL with 85% porosity and 300-500 μm pore size. A mixture of bovine chondrocytes and chitosan-alginategel was seeded and compared with alginate as a control on the PLCL scaffold. The cell maturation, proliferation,extracellular matrix synthesis, glycosaminoglycans (sGAG) production and collagen type-II expressions were better inchondrocytes seeded in chitosan-alginate hydrogel than in alginate only. These results indicate that chondrocytes withchitosan-alginate gel on PLCL scaffolds provide an appropriate biomimetic environment for cell proliferation and matrixsynthesis, which could successfully be used for cartilage repair and regeneration.
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KEYWORD
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PLCL, bovine chondrocytes, cartilage repair
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